Background-subtracted data values (mean +/- SD) are graphed. Description 1. After preparation, wrap in foil (see Note 1). The chloride and pH. 1. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). 383 inches whereas the maximum allowed is 5. Fluorescence Microplate Reader Comparison Chart. What do I do now? In our tests, the Greiner Bio-One Fluotrac™ 200 Microplate has shown lower background. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. iD3, iD5, i3, i3x, M3, M4, M5, M5e, L, and FlexStation 3 readers. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Bioz Stars score: 86/100, based on 1 PubMed citations. SpectraMax® Aquamax. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. 116 SpectraMax M5 and M5e Multi-Mode. Plus 384 ABS1 Location: Reader Validation-Plate Abs A reminder about recertification was added. The fluorescent signal was measured by multiplate reader (Spectramax M4, Molecular Devices LLC, Sunnyvale, California, USA). The UV‐Vis absorption spectra and cell viability were measured by a multi‐mode microplate reader (SpectraMax® M4, Molecular Devices LLC) using quartz cuvettes with 1‐cm path length (Starna Scientific Ltd. > spectramax m4 microplate reader. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini EM reader, SpectraMax M5/M5e reader, FlexStation 3 reader, SpectraMax M4 reader Which is better: top-read or bottom-read? You can use either. Large molecule bioanalysis, immunogenicity and PK testing with higher quality data, rapid turnaround and fewer surprises. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The polysaccharide HPA with different concentrations (0, 25, 50, and 100 μg/mL) was added and incubated with cells for 24 h. Preconfigured SoftMax ® Pro Software protocols (available at softmaxpro. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The optical systems Operated from within SoftMax Pro Software, the StakMax Microplate Handler can hold up to 50 plates and facilitates barcode reading. Cellular viability was assessed by using resazurin at 1. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Vol 4, Iss 22, Nov 20, 2014 . $1,250. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The conjugate was also characterized using FTIR (FT/IR-4200, Jasco Inc. , USA). The data represent the mean ± SD of three independent experiments. The incubation system pre-incubated at 37°C for 3 min minutes and 2 μL fluorescent substrate FD (5 μM, final concentration) was added for fluorescence analysis in fully automated fluorescent microplate reader (SpectraMax M4, Molecular Devices) (Wang et al. Quick, Simple Standardization: The new analyzer features SPECTRO’s proprietary iCAL 2. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Serum C-reactive protein (CRP) was quantified using the Mouse C-Reactive Protein/CRP Quantikine ELISA Kit. SpectraMax Gemini XPS/EM Register or Sign In. Table 2. The absorbance at 550 nm was recorded using a SpectraMax M4 microplate reader (Molecular Devices) following solvation of formazan with 100 μL DMSO. After 3 times washing, cells were added into 96-well plate and were examined under a fluorescence microplate reader (Excitation, 345 nm; Emission 495 nm) (SpectraMax M4 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA). Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. 4) and cells were stained with 150 nM TSQ for 1 min. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. The M3/M4/M5/M5e/Flex3 and the L/LMax II/LMax II 384 groups of readers each require different Dead Time Correction values, and therefore each has their own Certification. Products : SpectraMax M3 reader, SpectraMax M4 reader, SpectraTest Validation Plates LM1 , FlexStation 3. . Background-subtracted data values (mean +/- SD) are graphed. Serving Victoria's renovation and construction needs. 3. The SpectraMax M5e reader offers the additional benefit of being certified . The fluorescence intensity was measured as ratio of values at an excitation at 340 nm and 380 nm with an emission at 510 nm, using Spectramax M4 (Molecular Devices). Choose from a three- (M3), four- (M4), or Cables. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. 5 pM 384-well (50 µL) 6 pM 10 pM 1536-well (8 µL) 19 pM 25 pM 0200-7015 (LUM 384) Optimized Guaranteed* 96-well (200 µL) 1. free. Diclofenac potassium at non-toxic concentration on U87-MG was used as positive control. Measurement of Mitochondrial Membrane Potential by JC-1. The IC50 curves were calculated using variable slope for log (inhibitor) vs. Walter2, and Roch-Philippe Charles1 1Institute for Biochemistry and Molecular. Preconfigured SoftMax ® Pro Software protocols (available at softmaxpro. The medium was then removed from the wells, and 100 µl of DMSO was added to solubilize the formazan crystals. StakMax Handling Stacker Base Unit. Simulated Exposure SystemOptical density readings were obtained at various timepoints from 0 to 128 hours following inoculation using a SpectraMax M4 spectrophotometer (Molecular Devices, San Jose, CA). Data are from ≥5 independent experiments. org) with optimized instrument settings (Table 1) were used to generate and analyze data. Background-subtracted data values (mean +/- SD) are graphed. Western blot apparatus (BIO-RAD Laboratories). The ELISA kits (Lianke Bio, China) were used to detect the concentration of specific cytokines. Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. book Page 1 Wednesday, July 7, 2010 2:09 PM SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. Spectramax M Series, supplied by Danaher Inc, used in various techniques. Firefly luciferase, a 61 kD monomeric protein, is especially attractive to many researchers because of its high sensitivity, wide linear detection range and extremely low background due to the absence of endogenous luminescent activity in mammalian cells. SPECTROMAXx with iCAL 2. Rhizosphere soil microbial communities substantially impact plant growth by regulating the nutrient cycle. Absorbance was read at 570 nm with SpectraMax M4 microplate spectrophotometer (Molecular Devices, CA). 1. Moving a SpectraMax Multi-Mode Microplate Reader. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. SpectraMax® M4. 1 Figure from 528 Publications Molecular Devices LLC. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, AquaMax washer, SpectraMax Plus 384 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax L reader, SpectraMax M5/M5e reader. * Nunc. Background-subtracted data values (mean +/- SD) are graphed. Description 1. 05 mg mL −1. Home; Lighting; Work lights; User manual. High-performance liquid chromatography (HPLC) chromatograms were performed on Agilent Infinity 1260 with a C18 column (100 Å, 30 × 4. 1 mg·mL −1 (Sigma-Aldrich, USA) as described above and fluorescence intensity at λ ex = 530 nm and λ em = 590 nm was detected through a microplate reader (Spectramax M4, Molecular Devices, San Jose, CA, USA). 4. SpectraMax® M5. 1. Dettmer4, Wayne A. Table 1. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. iD3, iD5, i3, i3x, M3, M4, M5, M5e, L, and FlexStation 3 readers. 314 low lamp level warning. Multi-Mode Microplate Readers. Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. 2 only) SpectraMax M-series Microplate readers. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. SpectraMax ABS/ABS Plus SpectraMax i3/i3x MiniMax SpectraMax iD3/iD5 Spectramax 190 EXT Spectramax 340PC/384 Spectramax Plus 384 Spectramax L Spectramax M2 Spectramax M2e Spectramax M3 Spectramax M4 Spectramax M5 Spectramax M5e Spectramax Mini Versamax EXT Vmax Microplate Washers;. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Three kits covering a wide range of dsDNA concentrations. The OD value of each well was determined using a SpectraMax M4 Multimode Microplate reader (Molecular Devices, USA), and the concentration of cytokines was calculated by comparing their OD value with the corresponding standard curve. 3 or greater. 4 Cell viability assay The colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was employed to determine cell viability; 1. , contact Molecular. 1. 0 compensates for most changes in environmental temperature or pressure. Table 2. Examples: “Press carefully to engage,” “ Do not. 4. NCI-60 cell panel screening was performed by the NCI/National Institutes of Health developmental therapeutics program (Bethesda, US). Choose from a three- (M3), four- (M4), orThe absorbance of the biofilm was measured at 595 nm using a microplate reader (SpectraMax M4) and the results were expressed as percentage changes with respect to the control (without compounds). 4) and cells were stained with 150 nM TSQ for 1 min. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4-Dual monochromatic optics -Wide range of applications -Top or bottom read modes The SpectraMax M3 Multi-Mode Microplate Reader provides three modes of detection in one platform The SpectraMax M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. S. The SpectraMax M2 and M2e are versatile and reliable. Choose from a three- (M3), four- (M4), orLuciferase Detection Instrumentation Guide. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. 2. The whole process was protected from direct light. 3. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. 161 likes. When using a SpectraMax M3 Reader, the GxP audit trail incorrectly reported the instrument as a SpectraMax M4 Read er. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. 306 plate air cal fail,low light. When a feature or capability applies to only certain readers, this exception is noted. Molecular Devices SpectraMax ® M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. TEM. DNA standards. 1, BIO-RAD Laboratories). Optical density readings were obtained at various timepoints from 0 to 128 hours following inoculation using a SpectraMax M4 spectrophotometer (Molecular Devices, San Jose, CA). 1% CF3COOH) = 5/95 (v/v) to 95/5 (v/v); flow rate:The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. Phillips5, Martin A. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). In vitro transcription (IVT) is an essential technique for RNA synthesis. The drawing shows a standard microplate of 15 mm height covered with a lid. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThe SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. SpectraMax ABS Plus SpectraTest ABS2 v1. Choose from a three- (M3), four- (M4), orThe integration time suggested is 5-10 sec. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Colony formation assays were performed to measure cell proliferation. Cell viability was calculated using the formula as follows: (OD450 KO HepG2 -OD450 blank /OD450 WT HepG2 -OD450 blank ) × 100%. 2. All luciferase assays were conducted in CORNING ® TypeI Collagen Coated 96-Well Cell Culture Clear Bottom White plates. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. C. M4 Contracting Ltd, Victoria, British Columbia. Size and zeta potential of nanoparticles were measured by Zetasizer Nano ZS90 (Malvern Instrument). Statistical. +1 250-415-0785. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. . book Page 1 Wednesday, July 7, 2010 2:09 PM. org) with optimized instrument settings (Table 1) were used to generate and analyze data. The guide also covers the software features, the specifications, and the accessories of the systems. Microplate Reader Shelf. For the quantification of the number of viable bacteria in the biofilm, the biofilms were gently destroyed and plated on TSA after serial. Exhibit with us {{>currencyLabel}} Back. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. 3752 in as per SBS dimensions. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. . 315 can't find zero order. The OD value of each well was determined using a SpectraMax M4 Multimode Microplate reader (Molecular Devices, USA), and the concentration of cytokines was calculated by comparing their OD value with the corresponding standard curve. Shop our huge selection of OEM, MRO, construction, industrial, and safety products. However, traditional polyacrylamide gel electrophoresis (PAGE) and high. neoformans was cultured from glycerol stock in 5 mL of YM broth at 25°C for 72 hours at 200 rpm. Cells were stained with dichlorofluorescein diacetate, fixed, and immediately analyzed using a multianalytic validation system (SpectraMax M4). Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, AquaMax washer, SpectraMax Plus 384 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax L reader, SpectraMax M5/M5e. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. book Page 1 Wednesday, July 7, 2010 2:09 PM M4 SpectraMax M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. 8. When the user applies a set temperature of 37°C, the top is heated to 37. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Bio-layer interferometry assays The binding kinetics of 1H1 to spike or RBD proteins from SARS-CoV-2 variants was performed by biolayer interferometry analysis on the GatorPrime Label-Free Bioanalysis. Ltd. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Large Molecule Bioanalysis. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Bottom-reading is usually recommended for cell-based assay with adherent cells. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. . The test conditions are as follows: mobile phase: acetonitrile/H2O (0. 304 signal level saturation. book Page 1 Wednesday, July 7, 2010 2:09 PMLuciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. For the microspheres containing moxifloxacin used in vivo, the liquid hydrogel was presoaked in a 24 mg/mL aqueous solution of moxifloxacin hydrochloride. SpectraMax® Paradigm® Multi-Mode Detection Platform. Analysis of flow cytometric samples was performed with BD FACSDiva software (BD Biosciences, San Jose, CA). Devices SpectraMax® M4). 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forSpectraMax® M3. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate Reader The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Absorbance was measured at 570 nm using a multimode reader (Spectramax M4, USA) (Kalvala et al. data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Optimized for fluorescence microplate readers. Molecular Devices SpectraMax M4 upgradable microplate/cuvette reader (90V to 240V) with current version of SoftMax Pro. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. B07F4876MS. Five hours prior to when permeability will be measured, gavage mice with 0. The available phosphorus in the soil was leached by double-acid extraction (0. 4. Results were expressed as the mean percentage of CV compared with NC group. 11. . The pathophysiology of such. 2. . Table of Contents. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 1. Establishment of mouse orthotopic xenograft bladder tumor model. All of the experiments were performed in triplicate, and the results were represented as mean. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Subsequently, 20 µl CCK-8 solution (5 mg/ml) diluted in PBS was added to each well for another 1 h and the absorbance was measured at 450 nm using a SpectraMax M4 microplate reader (Molecular Devices, LLC). com. Plot the fluorescence intensity versus the cell seeding number. All associated StakMax® stackers. Table 2. Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) Total RNA was extracted at 48 h after exposure to volasertib, genistein, or gefitinib and quantified by a NanoDrop spectrophotometer (Thermo Scientific; Wilmington, DE, USA). Immunodetection Protocols for Cell Culture. Titration of human serum and plasma (heparin) within the working range of the assay. In the M2, M5 and Plus. At 48 h after transfection, the Nano-Glo Live Cell Assay reagent was added and the luciferase activity in the medium and cells were measured using a SpectraMax M4 (Molecular Devices, San Jose, CA, USA). All experiments. Steady-state kinetics measurements were performed at 25°C in 96-well plates (Corning) using a SpectraMax M4 spectrophotometer (Molecular Devices) in PBS buffer. Inhibition of CPT-11 Hydrolysis by Four Bioflavonesfor the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. 5. SpectraMax® M4). The content was vortexed for 1 min and incubated in the dark for 20 min at 25°C. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Sandwich ELISA - Human TNF alpha ELISA Kit, Fluorescent (ab229399) To learn more about the advantages of recombinant antibodies see here. $2,219. The fluorescence intensity was measured as ratio of values at an excitation at 340 nm and 380 nm with an emission at 510 nm, using Spectramax M4 (Molecular Devices). for Cisbio Bioassays. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. 7°C (gradient heating design). Microplate Reader Shelf. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. FlexStation™ II. ITEM#: M4-MOL Molecular Devices SpectraMax M4 upgradable microplate/cuvette reader (90V to 240V) with current version of SoftMax Pro. 025 mol/L sulfuric acid) and then measured following the molybdenum antimony colorimetric method (multifunctional microplate reader, SpectraMax M4, MD company, USA). SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. The use of gene reporters such as luciferase permits highly sensitive and nondestructive monitoring of gene expression. 90-0083 series): Excitation 530 nm,. C. spr * SpectraMax i3(x) SpectraTest ABS2 v1. The final concentration of hemoglobin in feces was. These samples were analyzed using a multimode plate reader (SpectraMax M4, Molecular Devices, USA). The enzymatic reaction was triggered by addition of luminol and the chemiluminescence signal was monitored at 37 °C using a microplate reader SpectraMax M4 (Molecular Devices, Sunnyvale, CA, USA), with one acquisition every 2 s for 300 min. 5 mg mL −1 with 0. The SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. Images were taken using a fluorescence microscope (Nikon 80i, Japan). The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. . Like all. , and red fluorescence at 543 ex. Lastly, the TMB substrate (Beyotime) was added, and absorbance was measured at 450 nm by a microplate reader (SpectraMax M4). SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Published Dec 16, 2020 | Updated Dec 16, 2020. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. alamarBlue reagent volume in a different spheroid microplate formatA SpectraMax M4 microplate reader (Molecular Devices, LLC, Sunnyvale, CA, USA) was used to measure the absorbance at a wavelength of 450 nm. View specifications, prices, citations, reviews, and more. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. Background-subtracted data values (mean +/- SD) are graphed. VIEW DETAILS The samples were centrifuged to pellet the remaining intact erythrocytes, and supernatants were collected. The adherent cells were incubated in 100 μl of lysis buffer on ice for 20 min. My List. Note: In this user guide, all references to SpectraMax Multi-Mode. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. 1 & 7. Cells were exposed to drug solutions with. 1. Standard features include a cuvette port,. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. The absorption value at 570 nm was measured using a Molecular Devices SpectraMax M4 plate reader, and the absorbance at 690 nm was subtracted as the background. Standard curves for quantifying samples were generated by using serial dilutions of BSA from 0 to 0. Residual. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politicsSpectraMax Spectrofluorometers. 3, La Jolla, CA, USA). New PBS 1X was added into each well and oxidized DCFH excitation and emission were measured at 485 and 525 nm respectively in a spectrophotometer (SpectraMax M4, Molecular Devices, Sunnyvale, USA). Description 1. Dagda Lab. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384-well microplates. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from. The optical density at 450 nm was detected using a microplate reader (SpectraMax M4, MolecularDevices, CA, USA). Fastenal is the largest fastener distributor in North America. Lower limits of detection for ATP determined using the ATPlite . Figure 1. Molecular Devices. Hemoglobin release from erythrocyte lysis was detected by measuring the optical density (OD) of the supernatant at 412 nm using a microplate reader (SpectraMax M4 from Molecular Devices, or Tecan SPARK, from Tecan, Männedorf,. 50 μl of samples were added into the mixture of 40 μl of detection buffer and 10 μl of Ac-DEVD-pNA (2 mM) with gentle shaking for 5 min. Technical specifications Detection limit for ATP Glow 0200-7012 (LUM) Optimized Guaranteed* 96-well (200 µL) 1. This assay kit is optimized for the SpectraMax i3x Multimode Microplate Reader with a preconfigured protocol in SoftMax Pro Software for rapid. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. We therefore gathered salient. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. Products : SpectraMax M2/M2e reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax 190 reader, VersaMax reader, Gemini EM reader, SpectraMax Plus 384 reader. In. Background-subtracted data values (mean +/- SD) are graphed. All configurations. Other technologies such as imaging. Control AM were incubated in the same buffer for 1 h at 37 °C. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. D 1 1. D 47 7. Pierce™ BCA Protein Assay Kit was purchased from Thermo Fisher Scientific Inc. org) with optimized instrument settings (Table 1) were used to generate and analyze data. The plate reader optics are tunable for absorbance. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. In the competitive field of drug development where timely go/no-go decisions must be made to bring effective class-leading drugs to market, it is vital to work with a CRO that can provide highly. Molecular Devices LLC spectramax m4 multimode microplate reader page 12 25 Spectramax M4 Multimode Microplate Reader Page 12 25, supplied by Molecular Devices LLC, used in various techniques. FD-4 concentrations in plasma were analyzed in duplicates using a spectrophotometer (SpectraMax M4, Molecular Devices, San Jose, CA, USA) with excitation λ 485 nm and emission λ 535 nm. Description 1. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. 116 SpectraMax M5 and M5e Multi-Mode. At sacrifice, serum was isolated from mouse total blood and maintained at –80 °C. MTT values at day 3 were normalized to day 0. 5 mL of creatinine, FD-4, and rhodamine70 dissolved in. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. SpectraMax M4 Multi-Mode Microplate Reader. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Preconfigured SoftMax® Pro Software protocols (available at softmaxpro. † Bottom mounting for SpectraMax Paradigm Readers only. ELISA standard curves were generated using a four-parameter logistic curve model (GraphPad Prism 8, GraphPad Software; San Diego, CA, USA). 0. Ninety microliters of 2-chloro-4-nitrophenyl-α- d -maltotrioside substrate dilutions from 5000 to 1. All configurations. We therefore gathered salient information about many of the. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected]. F1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. 2. Finally, we have two New Brunswick Excella E24 shaking incubators, one with refrigeration capabilities and a Thermo Sciences -80˚C freezer. Samples (2 mg) were mixed with potassium bromide in a 1:100 ratio before the analysis. 1. SpectraMax M3 Multi-Mode Microplate Reader with 1-Year Service Contract. 1 & 7. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. High tolerance for contaminants. normalized response (Graphpad Prism 8. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. 5. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, SpectraMax M4 reader, SpectraMax M3 reader MTT reduction - a tetrazolium-based colorimetric assay for cell survival and proliferation (Maxline Application Note #5) (PDF) Summary: The MTT assay measures cell respiration and the amount of formazan. . Add To My List; Create New List; Quickview. 2. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Z’-LYTE® Assay using the Z'-LYTE® Tyr6 kit. The fluorescent signal at an excitation wavelength of 530 nm and emission wavelength of 580 nm was measured by Spectramax M4, USA. Highly selective for dsDNA over ssDNA or RNA. Optimized for fluorescence microplate readers. The IC50 curves were calculated using variable slope for log (inhibitor) vs. Cleavage of the fluorescent NAG substrate (4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-Glucopyranoside) was measured for 20 min with 45 s intervals at 30°C in a Molecular Devices (San Jose, CA) SpectraMax M4 microplate reader. A series of Toll-like receptor 7 (TLR7)-specific antagonists and extensive structural analysis reveal the open conformation of the receptor and the structural basis of TLR7 antagonism. What types of plates can be used with StakMax? SBS Standard 96 and 384 well plates with plate height of 12-16 mm are recommended. Description 1. SpectraMax Paradigm SpectraMax i3 SpectraMax Flex-Station 3 SpectraMax F5 SpectraMax M4, M5, and M5e SpectraMax M2, and M2e: Secondary modes: BMG LABTECH: CLARIOstar PHERAstar FS PHERAstar Plus FLUOstar Omega POLARstar Omega PerkinElmer: EnSpire® Multimode Plate Reader EnVision Multilabel Plate. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. After 48 h, the optical density (OD) was measured at 450 nm using the microplate reader (SpectraMax M4). ATP standard curves read on SpectraMax microplate readers. For heme titration experiments a constant ATP concentration of 1 mM was used. Standard curves for quantifying samples were generated by using serial dilutions of BSA from 0 to 0. Find M4 Group Marketing Specialists in Victoria, with phone, website, address, opening hours and contact info. 10. item 7 replacement bulb for molecular devices spectramax m4 flash lamp replacement bulb for molecular devices spectramax m4 flash lamp. Description 1. ISM Equipment Classification (Group 1, Class A) This equipment is. • Tel. Real time thermal cyclers (iCycleriQ apparatus with iCyclerOptical System software version 3. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. es • Flexibility for your assay needs: Robotics compatibility for increased throughput:すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. We show that a ML model, trained on the. For labeling representative cells, they were treated under the same conditions as above, followed by fixation with 2% paraformaldehyde for 20 min at room temperature. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME.